Identification of wild yeast species by DNA sequencing

Applications

Wild yeast can be difficult to identify as traditional methods for yeast identification are often labor intensive and lack precision. Our DNA sequencing process allows for the accurate identification of isolated yeast contaminants to the species level including an expansive range of wild yeast strains associated with the food and beverage industry.

Technology

Sequencing of the D1-D2 domain within yeast ribosomal DNA can be used to rapidly and accurately identify yeast species. This method involves the amplification of rDNA by PCR followed by sequencing of the resulting fragment. Identification of yeast species is performed by comparison to a Basic Local Alignment Search Tool (BLAST) database of wild yeast strains common in the food and beverage industries.